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selective and sensitive bioprobe  (Axolabs Inc)

 
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    Axolabs Inc selective and sensitive bioprobe
    Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific <t>bioprobe</t> ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.
    Selective And Sensitive Bioprobe, supplied by Axolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/selective and sensitive bioprobe/product/Axolabs Inc
    Average 90 stars, based on 1 article reviews
    selective and sensitive bioprobe - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Antisense Oligonucleotide in LNA-Gapmer Design Targeting TGFBR2—A Key Single Gene Target for Safe and Effective Inhibition of TGFβ Signaling"

    Article Title: Antisense Oligonucleotide in LNA-Gapmer Design Targeting TGFBR2—A Key Single Gene Target for Safe and Effective Inhibition of TGFβ Signaling

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21061952

    Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific bioprobe ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.
    Figure Legend Snippet: Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific bioprobe ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.

    Techniques Used: Incubation, Quantitative RT-PCR, Western Blot, Comparison

    In-use stability of lead candidate X07091 = NVP-13. ( A ) Integrity of NVP-13 in 0.9 % NaCl (saline) when incubated with different temperature conditions and for different time intervals. For incubation at −20 °C +/− 5 °C a denaturizing Ion-Pair-Reversed-Pair High Performance Liquid Chromatography (IP-RP-HPLC) with Electrospray-Ionization (ESI)/Mass Spectrometry (MS) was used for determination of relative purity and identity of NVP-13. Aliquots incubated at 5 °C +/− 3 °C were analyzed using an IP-RP-HPLC combined with UV/Mass spectrometry and samples incubated at 37 °C (37 °C as adjusted by the integrated thermostat of New Brunswick Galaxy 170 S Incubator, Eppendorf) were analyzed using IP-RP-UPLC with UV/ESI/MS. Results showed that NVP-13 content was stable under all tested conditions. Discrepancy for content and purity/integrity were within method variability. Values are given as mean and SEM. Determination of intact NVP-13 with a specific bioprobe in the supernatant of cell culture media supernatant of ReNcell CX ® ( B ) and A549 cells ( C ) after incubation with 10 µM NVP-13 for up to 72 h, n = 3. Line of best fit for ( B ) and ( C ) was calculated by a nonlinear fit function.
    Figure Legend Snippet: In-use stability of lead candidate X07091 = NVP-13. ( A ) Integrity of NVP-13 in 0.9 % NaCl (saline) when incubated with different temperature conditions and for different time intervals. For incubation at −20 °C +/− 5 °C a denaturizing Ion-Pair-Reversed-Pair High Performance Liquid Chromatography (IP-RP-HPLC) with Electrospray-Ionization (ESI)/Mass Spectrometry (MS) was used for determination of relative purity and identity of NVP-13. Aliquots incubated at 5 °C +/− 3 °C were analyzed using an IP-RP-HPLC combined with UV/Mass spectrometry and samples incubated at 37 °C (37 °C as adjusted by the integrated thermostat of New Brunswick Galaxy 170 S Incubator, Eppendorf) were analyzed using IP-RP-UPLC with UV/ESI/MS. Results showed that NVP-13 content was stable under all tested conditions. Discrepancy for content and purity/integrity were within method variability. Values are given as mean and SEM. Determination of intact NVP-13 with a specific bioprobe in the supernatant of cell culture media supernatant of ReNcell CX ® ( B ) and A549 cells ( C ) after incubation with 10 µM NVP-13 for up to 72 h, n = 3. Line of best fit for ( B ) and ( C ) was calculated by a nonlinear fit function.

    Techniques Used: Saline, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Cell Culture



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    selective and sensitive bioprobe  (Axolabs Inc)
    90
    Axolabs Inc selective and sensitive bioprobe
    Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific <t>bioprobe</t> ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.
    Selective And Sensitive Bioprobe, supplied by Axolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/selective and sensitive bioprobe/product/Axolabs Inc
    Average 90 stars, based on 1 article reviews
    selective and sensitive bioprobe - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific bioprobe ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.

    Journal: International Journal of Molecular Sciences

    Article Title: Antisense Oligonucleotide in LNA-Gapmer Design Targeting TGFBR2—A Key Single Gene Target for Safe and Effective Inhibition of TGFβ Signaling

    doi: 10.3390/ijms21061952

    Figure Lengend Snippet: Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific bioprobe ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.

    Article Snippet: After harvest, the supernatant was collected, cells were counted and both, cell culture media supernatant and cell pellets, were assessed for their X07091 = NVP-13 content at Axolabs GmbH (Kulmbach, Germany) using a selective and sensitive bioprobe.

    Techniques: Incubation, Quantitative RT-PCR, Western Blot, Comparison

    In-use stability of lead candidate X07091 = NVP-13. ( A ) Integrity of NVP-13 in 0.9 % NaCl (saline) when incubated with different temperature conditions and for different time intervals. For incubation at −20 °C +/− 5 °C a denaturizing Ion-Pair-Reversed-Pair High Performance Liquid Chromatography (IP-RP-HPLC) with Electrospray-Ionization (ESI)/Mass Spectrometry (MS) was used for determination of relative purity and identity of NVP-13. Aliquots incubated at 5 °C +/− 3 °C were analyzed using an IP-RP-HPLC combined with UV/Mass spectrometry and samples incubated at 37 °C (37 °C as adjusted by the integrated thermostat of New Brunswick Galaxy 170 S Incubator, Eppendorf) were analyzed using IP-RP-UPLC with UV/ESI/MS. Results showed that NVP-13 content was stable under all tested conditions. Discrepancy for content and purity/integrity were within method variability. Values are given as mean and SEM. Determination of intact NVP-13 with a specific bioprobe in the supernatant of cell culture media supernatant of ReNcell CX ® ( B ) and A549 cells ( C ) after incubation with 10 µM NVP-13 for up to 72 h, n = 3. Line of best fit for ( B ) and ( C ) was calculated by a nonlinear fit function.

    Journal: International Journal of Molecular Sciences

    Article Title: Antisense Oligonucleotide in LNA-Gapmer Design Targeting TGFBR2—A Key Single Gene Target for Safe and Effective Inhibition of TGFβ Signaling

    doi: 10.3390/ijms21061952

    Figure Lengend Snippet: In-use stability of lead candidate X07091 = NVP-13. ( A ) Integrity of NVP-13 in 0.9 % NaCl (saline) when incubated with different temperature conditions and for different time intervals. For incubation at −20 °C +/− 5 °C a denaturizing Ion-Pair-Reversed-Pair High Performance Liquid Chromatography (IP-RP-HPLC) with Electrospray-Ionization (ESI)/Mass Spectrometry (MS) was used for determination of relative purity and identity of NVP-13. Aliquots incubated at 5 °C +/− 3 °C were analyzed using an IP-RP-HPLC combined with UV/Mass spectrometry and samples incubated at 37 °C (37 °C as adjusted by the integrated thermostat of New Brunswick Galaxy 170 S Incubator, Eppendorf) were analyzed using IP-RP-UPLC with UV/ESI/MS. Results showed that NVP-13 content was stable under all tested conditions. Discrepancy for content and purity/integrity were within method variability. Values are given as mean and SEM. Determination of intact NVP-13 with a specific bioprobe in the supernatant of cell culture media supernatant of ReNcell CX ® ( B ) and A549 cells ( C ) after incubation with 10 µM NVP-13 for up to 72 h, n = 3. Line of best fit for ( B ) and ( C ) was calculated by a nonlinear fit function.

    Article Snippet: After harvest, the supernatant was collected, cells were counted and both, cell culture media supernatant and cell pellets, were assessed for their X07091 = NVP-13 content at Axolabs GmbH (Kulmbach, Germany) using a selective and sensitive bioprobe.

    Techniques: Saline, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Cell Culture